Journal: eLife

Article Title: Multicolor fluorescence fluctuation spectroscopy in living cells via spectral detection

doi: 10.7554/eLife.69687

Figure Lengend Snippet: ( A–C ) Representative correlation functions (CFs) (green/yellow/red: autocorrelation functions [ACFs] for mEGFP [‘G’]/mEYFP [‘Y’]/mCherry2 [‘Ch2’]; purple/blue/gray: cross-correlation functions [CCFs] calculated for the pairs mEGFP and mEYFP/mEGFP and mCherry2/mEYFP and mCherry2) obtained from three-species SFSCS measurements on HEK 293T cells co-expressing mp-mEGFP, mp-mEYFP, and mCherry2 ( A ), mp-mCherry2-mEGFP heterodimers and mp-mEYFP ( B ), or expressing mp-mEYFP-mCherry2-mEGFP heterotrimers ( C ), as illustrated in insets. Solid thick lines show fits of a two-dimensional diffusion model to the CFs. ( D ) Representative fluorescence images of HEK 293T cells co-expressing CD9-mEGFP, LC3-mEYFP, and IAV protein M2-mCh2. Spectral filtering and decomposition were performed to obtain a single image for each species. Scale bars are 5 µm. ( E ) Representative CFs (green/yellow/red: ACFs for mEGFP/mEYFP/mCherry2; purple/blue/gray: CCFs calculated for the pairs mEGFP and mEYFP/mEGFP and mCherry2/mEYFP and mCherry2) obtained from three-species SFSCS measurements on HEK 293T cells co-expressing CD9-mEGFP, LC3-mEYFP, and M2-mCh2. Solid thick lines show fits of a two-dimensional diffusion model to the CFs. ( F ) Relative cross-correlation values obtained from three-species SFSCS measurements described in ( A–C ) and ( E ). ( G ) Normalized molecular brightness values obtained from three-species SFSCS measurements on HEK 293T cells co-expressing mp-mEGFP, mp-mEYFP, and mp-mCherry2 (blue), mp-2x-mEGFP, mp-mEYFP, and mp-mCherry2 (red), CD9-mEGFP, LC3-mEYFP, and M2-mCh2 (green), or expressing mp-mEGFP alone (yellow). Normalized brightness values were calculated by dividing the molecular brightness values detected in each SFSCS measurement by the average brightness obtained for mEGFP, mEYFP, and mCherry2 in cells co-expressing mp-mEGFP, mp-mEYFP, and mp-mCherry2. Data are pooled from two independent experiments for each sample. The number of cells measured is given in parentheses. Error bars represent mean ± SD. Figure 3—source data 1. Relative cross-correlation and normalized molecular brightness values for three-species scanning fluorescence correlation spectroscopy measurements.

Article Snippet: The CD9-mEGFP plasmid was cloned by amplifying CD9 from pCMV3-CD9 (obtained from SinoBiological #HG11029-UT, encoding human CD9) and inserting into mEGFP-C1 vector using restriction by HindIII and BamHI.

Techniques: Expressing, Diffusion-based Assay, Fluorescence, Spectroscopy

Journal: The Cochrane Database of Systematic Reviews

Article Title: Rapid diagnostic tests for diagnosing uncomplicated P. falciparum malaria in endemic countries

doi: 10.1002/14651858.CD008122.pub2

Figure Lengend Snippet: RDT types and brands verified with microscopy

Article Snippet: Target condition and reference standard(s) Target condition: Malaria parasitaemia Reference standard: Microscopy thick and thin blood films Person(s) performing microscopy: Trained malaria technician Microscopy setting: Not stated Number of high power fields examined before declaring negative: 200 Number of observer or repeats: One, except in the case of discordant results between microscopy and RDTs Resolution of discrepancies between observers: All discordant results, all slides where only gametocytes were detected and a random sample of 20% of the remaining slides were checked blind by an independent trained laboratory technician Index and comparator tests Commerical name of RDTs: Paracheck Pf dipstick (Orchid Biomedical Systems, Goa, India) Paracheck Pf device (Orchid Biomedical Systems, Goa, India) (data not included in review as duplicates Paracheck Pf dipstick) ParaHIT‐f (Span diagnostics Ltd, Surat, India) (excluded as required data could not be extracted) BIO P.F (excluded as required data could not be extracted) Malaria Rapid (excluded as required data could not be extracted) Parasite(s) designed to detect: P. falciparum Designated Type: Type 1 Batch numbers: Not stated Transport and storage conditions: Not described Person(s) performing RDT: Two independent readers (persons unspecified) RDT setting: The research clinic Follow‐up Not applicable Notes Source of funding: Medicins sans Frontieres, French section.

Techniques:

Journal: The Cochrane Database of Systematic Reviews

Article Title: Rapid diagnostic tests for diagnosing uncomplicated P. falciparum malaria in endemic countries

doi: 10.1002/14651858.CD008122.pub2

Figure Lengend Snippet: New Summary of results table

Article Snippet: Target condition and reference standard(s) Target condition: Malaria parasitaemia Reference standard: Microscopy thick and thin blood films Person(s) performing microscopy: Trained malaria technician Microscopy setting: Not stated Number of high power fields examined before declaring negative: 200 Number of observer or repeats: One, except in the case of discordant results between microscopy and RDTs Resolution of discrepancies between observers: All discordant results, all slides where only gametocytes were detected and a random sample of 20% of the remaining slides were checked blind by an independent trained laboratory technician Index and comparator tests Commerical name of RDTs: Paracheck Pf dipstick (Orchid Biomedical Systems, Goa, India) Paracheck Pf device (Orchid Biomedical Systems, Goa, India) (data not included in review as duplicates Paracheck Pf dipstick) ParaHIT‐f (Span diagnostics Ltd, Surat, India) (excluded as required data could not be extracted) BIO P.F (excluded as required data could not be extracted) Malaria Rapid (excluded as required data could not be extracted) Parasite(s) designed to detect: P. falciparum Designated Type: Type 1 Batch numbers: Not stated Transport and storage conditions: Not described Person(s) performing RDT: Two independent readers (persons unspecified) RDT setting: The research clinic Follow‐up Not applicable Notes Source of funding: Medicins sans Frontieres, French section.

Techniques: Diagnostic Assay, Microscopy, Sampling

Journal: The Cochrane Database of Systematic Reviews

Article Title: Rapid diagnostic tests for diagnosing uncomplicated P. falciparum malaria in endemic countries

doi: 10.1002/14651858.CD008122.pub2

Figure Lengend Snippet: Paracheck‐Pf.

Article Snippet: Target condition and reference standard(s) Target condition: Malaria parasitaemia Reference standard: Microscopy thick and thin blood films Person(s) performing microscopy: Trained malaria technician Microscopy setting: Not stated Number of high power fields examined before declaring negative: 200 Number of observer or repeats: One, except in the case of discordant results between microscopy and RDTs Resolution of discrepancies between observers: All discordant results, all slides where only gametocytes were detected and a random sample of 20% of the remaining slides were checked blind by an independent trained laboratory technician Index and comparator tests Commerical name of RDTs: Paracheck Pf dipstick (Orchid Biomedical Systems, Goa, India) Paracheck Pf device (Orchid Biomedical Systems, Goa, India) (data not included in review as duplicates Paracheck Pf dipstick) ParaHIT‐f (Span diagnostics Ltd, Surat, India) (excluded as required data could not be extracted) BIO P.F (excluded as required data could not be extracted) Malaria Rapid (excluded as required data could not be extracted) Parasite(s) designed to detect: P. falciparum Designated Type: Type 1 Batch numbers: Not stated Transport and storage conditions: Not described Person(s) performing RDT: Two independent readers (persons unspecified) RDT setting: The research clinic Follow‐up Not applicable Notes Source of funding: Medicins sans Frontieres, French section.

Techniques:

Journal: The Cochrane Database of Systematic Reviews

Article Title: Rapid diagnostic tests for diagnosing uncomplicated P. falciparum malaria in endemic countries

doi: 10.1002/14651858.CD008122.pub2

Figure Lengend Snippet: ParaHIT‐F.

Article Snippet: Target condition and reference standard(s) Target condition: Malaria parasitaemia Reference standard: Microscopy thick and thin blood films Person(s) performing microscopy: Trained malaria technician Microscopy setting: Not stated Number of high power fields examined before declaring negative: 200 Number of observer or repeats: One, except in the case of discordant results between microscopy and RDTs Resolution of discrepancies between observers: All discordant results, all slides where only gametocytes were detected and a random sample of 20% of the remaining slides were checked blind by an independent trained laboratory technician Index and comparator tests Commerical name of RDTs: Paracheck Pf dipstick (Orchid Biomedical Systems, Goa, India) Paracheck Pf device (Orchid Biomedical Systems, Goa, India) (data not included in review as duplicates Paracheck Pf dipstick) ParaHIT‐f (Span diagnostics Ltd, Surat, India) (excluded as required data could not be extracted) BIO P.F (excluded as required data could not be extracted) Malaria Rapid (excluded as required data could not be extracted) Parasite(s) designed to detect: P. falciparum Designated Type: Type 1 Batch numbers: Not stated Transport and storage conditions: Not described Person(s) performing RDT: Two independent readers (persons unspecified) RDT setting: The research clinic Follow‐up Not applicable Notes Source of funding: Medicins sans Frontieres, French section.

Techniques:

Journal: The Cochrane Database of Systematic Reviews

Article Title: Rapid diagnostic tests for diagnosing uncomplicated P. falciparum malaria in endemic countries

doi: 10.1002/14651858.CD008122.pub2

Figure Lengend Snippet: PCR adjusted microscopy, Type 1, Paracheck‐PF (All).

Article Snippet: Target condition and reference standard(s) Target condition: Malaria parasitaemia Reference standard: Microscopy thick and thin blood films Person(s) performing microscopy: Trained malaria technician Microscopy setting: Not stated Number of high power fields examined before declaring negative: 200 Number of observer or repeats: One, except in the case of discordant results between microscopy and RDTs Resolution of discrepancies between observers: All discordant results, all slides where only gametocytes were detected and a random sample of 20% of the remaining slides were checked blind by an independent trained laboratory technician Index and comparator tests Commerical name of RDTs: Paracheck Pf dipstick (Orchid Biomedical Systems, Goa, India) Paracheck Pf device (Orchid Biomedical Systems, Goa, India) (data not included in review as duplicates Paracheck Pf dipstick) ParaHIT‐f (Span diagnostics Ltd, Surat, India) (excluded as required data could not be extracted) BIO P.F (excluded as required data could not be extracted) Malaria Rapid (excluded as required data could not be extracted) Parasite(s) designed to detect: P. falciparum Designated Type: Type 1 Batch numbers: Not stated Transport and storage conditions: Not described Person(s) performing RDT: Two independent readers (persons unspecified) RDT setting: The research clinic Follow‐up Not applicable Notes Source of funding: Medicins sans Frontieres, French section.

Techniques: Microscopy

Journal: The Cochrane Database of Systematic Reviews

Article Title: Rapid diagnostic tests for diagnosing uncomplicated P. falciparum malaria in endemic countries

doi: 10.1002/14651858.CD008122.pub2

Figure Lengend Snippet: PCR, Type 1, ParaHIT‐F.

Article Snippet: Target condition and reference standard(s) Target condition: Malaria parasitaemia Reference standard: Microscopy thick and thin blood films Person(s) performing microscopy: Trained malaria technician Microscopy setting: Not stated Number of high power fields examined before declaring negative: 200 Number of observer or repeats: One, except in the case of discordant results between microscopy and RDTs Resolution of discrepancies between observers: All discordant results, all slides where only gametocytes were detected and a random sample of 20% of the remaining slides were checked blind by an independent trained laboratory technician Index and comparator tests Commerical name of RDTs: Paracheck Pf dipstick (Orchid Biomedical Systems, Goa, India) Paracheck Pf device (Orchid Biomedical Systems, Goa, India) (data not included in review as duplicates Paracheck Pf dipstick) ParaHIT‐f (Span diagnostics Ltd, Surat, India) (excluded as required data could not be extracted) BIO P.F (excluded as required data could not be extracted) Malaria Rapid (excluded as required data could not be extracted) Parasite(s) designed to detect: P. falciparum Designated Type: Type 1 Batch numbers: Not stated Transport and storage conditions: Not described Person(s) performing RDT: Two independent readers (persons unspecified) RDT setting: The research clinic Follow‐up Not applicable Notes Source of funding: Medicins sans Frontieres, French section.

Techniques:

Journal: The Cochrane Database of Systematic Reviews

Article Title: Rapid diagnostic tests for diagnosing uncomplicated P. falciparum malaria in endemic countries

doi: 10.1002/14651858.CD008122.pub2

Figure Lengend Snippet: Data tables by test

Article Snippet: Target condition and reference standard(s) Target condition: Malaria parasitaemia Reference standard: Microscopy thick and thin blood films Person(s) performing microscopy: Trained malaria technician Microscopy setting: Not stated Number of high power fields examined before declaring negative: 200 Number of observer or repeats: One, except in the case of discordant results between microscopy and RDTs Resolution of discrepancies between observers: All discordant results, all slides where only gametocytes were detected and a random sample of 20% of the remaining slides were checked blind by an independent trained laboratory technician Index and comparator tests Commerical name of RDTs: Paracheck Pf dipstick (Orchid Biomedical Systems, Goa, India) Paracheck Pf device (Orchid Biomedical Systems, Goa, India) (data not included in review as duplicates Paracheck Pf dipstick) ParaHIT‐f (Span diagnostics Ltd, Surat, India) (excluded as required data could not be extracted) BIO P.F (excluded as required data could not be extracted) Malaria Rapid (excluded as required data could not be extracted) Parasite(s) designed to detect: P. falciparum Designated Type: Type 1 Batch numbers: Not stated Transport and storage conditions: Not described Person(s) performing RDT: Two independent readers (persons unspecified) RDT setting: The research clinic Follow‐up Not applicable Notes Source of funding: Medicins sans Frontieres, French section.

Techniques: Microscopy